Lab One: Staining
Gram Stain
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Acid Fast Stain |
Negative Stain
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Positive Stain
|
Endospore Stain
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Capsule Stain
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Safety first:
1. Never leave flame unattended.
2. Tie back hair.
3. Turn off gas after use.
4. Keep flammable reagents (such as alcohol) away from flame.
5. Overheating a glass slide will cause it to break.
Procedure
for making a slide from a broth culture:
1. Always hold the slide by the edge to prevent making fingerprints
(even when wearing gloves).
2. Sterilize the inoculating loop by holding it in a flame
until it is red. Let the loop cool near the flame to prevent contamination.
3. Pass the lip of the test tube in the flame (hold the
cap in your hand).
4. Place a loopful of culture onto the slide.
5. Pass the lip of the test tube in the flame again and
then place the cap on the tube.
6. Let the slide air dry.
7. Then heat fix the slide by passing it over the flame
four times. Do NOT heat fix when performing
a capsule stain.
8. Let cool.
Procedure
for making a slide from a solid culture
1. Always hold the slide by the edge to prevent making fingerprints
(even when wearing gloves).
2. Sterilize the inoculating loop by holding it in a flame
until it is red. Let the loop cool near the flame to prevent contamination.
3. Place a loopful of water onto the slide.
4. Sterilize the inoculating loop by holding it in a flame
until it is red. Let the loop cool near the flame to prevent contamination.
5. Scrape a small amount of culture and place it in the
water on the slide.
6. Let the slide air dry
7. Then heat fix the slide by passing it over the flame
four times. Do NOT heat fix when performing
a capsule stain.
8. Let cool.
References: